Sticky ends from different BanII sites may not be compatible. Sticky ends from different Bsu36I sites may not be compatible.Įfficient cleavage requires at least two copies of the PluTI recognition sequence.Įfficient cleavage requires at least two copies of the NarI recognition sequence.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.īssHII is typically used at 50☌, but is 75% active at 37☌. Sticky ends from different BtgI sites may not be compatible. 1020) lac operator lac operator lac operator lac operator stop codons stop codons stop codons stop codons pGEX-2T 4948 bp 1020) pGEX 3' Sequencing Primer pGEX 3' Sequencing Primer (998. 891) pGEX 5' Sequencing Primer pGEX 5' Sequencing Primer (869.
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